Abstract
In the present study, bronchial Gq protein level of the airway hyperresponsive rats was determined by using immunoblot analysis. In the airway hyperresponsive rats that were sensitized and repeatedly antigen challenged, the in vitro bronchial responsiveness to acetylcholine was significantly enhanced as compared with that in the sensitized control group. Moreover, the bronchial contraction induced by 10 μM AlF 4 − (generated by 10 μM AlCl 3 plus 10 mM NaF) was significantly elevated after repeated antigen challenge (0.44±0.13 and1.09±0.09 g tension in the control and airway hyperresponsive groups, respectively; P<0.01). In both groups, immunoblotting with the antibody against Gαq gave a single 42 kD band. The Gαq protein levels in the airway hyperresponsive group (0.58±0.12) estimated by Gαq/β-actin ratio was significantly greater than those in the control group (0.30±0.10; P<0.05). These findings suggest that the increase in Gαq protein level may be involved in the pathogenesis of antigen-induced airway hyperresponsiveness in rats.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
