Abstract
B1 cells constitute a specialized subset of B cells, best characterized in mice, which is abundant in body cavities, including the peritoneal cavity. Through natural and antigen-induced antibody production, B1 cells participate in the early defense against bacteria. The G protein-coupled receptor 183 (GPR183), also known as Epstein-Barr virus-induced gene 2 (EBI2), is an oxysterol-activated chemotactic receptor that regulates migration of B cells. We investigated the role of GPR183 in B1 cells in the peritoneal cavity and omentum. B1 cells expressed GPR183 at the mRNA level and migrated towards the GPR183 ligand 7α,25-dihydroxycholesterol (7α,25-OHC). GPR183 knock-out (KO) mice had smaller omenta, but with normal numbers of B1 cells, whereas they had fewer B2 cells in the omentum and peritoneal cavity than wildtype (WT) mice. GPR183 was not responsible for B1 cell accumulation in the omentum in response to i.p. lipopolysaccharide (LPS)-injection, in spite of a massive increase in 7α,25-OHC levels. Lack of GPR183 also did not affect B1a- or B1b cell-specific antibody responses after vaccination. In conclusion, we found that GPR183 is non-essential for the accumulation and function of B1 cells in the omentum and peritoneal cavity, but that it influences the abundance of B2 cells in these compartments.
Highlights
B1 cells are a subset of B cells defined by their unique phenotypes and functions
We found that while B1 cells expressed G protein-coupled receptor 183 (GPR183) at the mRNA level and migrated towards 7α,25-OHC in vitro, GPR183 was dispensable for the accumulation and function of
CD5 in itself is not a B1 cell-specific marker, as it is expressed on anergic B cells in mice [34]
Summary
B1 cells are a subset of B cells defined by their unique phenotypes and functions. They are best characterized in mice, and in mouse spleens they make up only ~2% of B cells, 4.0/).whereas they are much more abundant in the pleural and peritoneal cavities [1,2,3]. B1 cells are a subset of B cells defined by their unique phenotypes and functions. They are best characterized in mice, and in mouse spleens they make up only ~2% of B cells, 4.0/). Whereas they are much more abundant in the pleural and peritoneal cavities [1,2,3]. The existence of, and identity of, human B1 cells is still controversial [4,5]. B1 cells can be distinguished from conventional (B2) B cells by their surface marker expression profile. B1 cells have a low surface expression of B220 The surface marker CD5 groups B1 cells into two subsets; B1a cells, which express CD5, and
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