Abstract
Modern research has found that Ganoderma lucidum polysaccharides (GLP) and Polyporus umbellatus polysaccharides (PUP) mainly exhibit immunoregulation. However, the immune function of a polysaccharide composition consisting of GLP and PUP has not been studied. In this study, we developed a polysaccharide composition consisting of GLP and PUP in a ratio of 3:1 (named GPP). The immunoregulation of GPP was detected in RAW264.7 macrophages. Then, the acute oral toxicity of GPP and the effect of GPP on immunoregulation in mice was detected. The results showed that GPP enhanced the function of macrophage RAW264.7 cells through improving phagocytic ability, nitric oxide (NO) production and the mRNA expression of inducible nitric oxide synthase (iNOS) and tumor necrosis factor (TNF)-α. GPP belonged to the non-toxic grade in mice. Moreover, GPP significantly improved macrophage phagocytic function and the activity of natural killer (NK) cells after being administered to mice at a dose of 0, 3.6, 120, 360 mg/kg body weight (mg/kg BW) orally for 30 days. Taken together, these findings suggested that GPP moderately regulated immune function in mice, which contributes to the further development and utilization of GLP and PUP in immune function.
Highlights
Polysaccharides, a class of natural macromolecule polymers which consist of more than 10 monosaccharides linked with glycosidic bonds, have a wide range of biological activities [1].Edible mushroom polysaccharides (EMPs) are a member of the polysaccharide family
The results showed that GPP enhanced the function of macrophages by improving phagocytic function, promoting NO production, and increasing the RNA expression of inducible nitric oxide synthase and tumor necrosis factor (TNF)-α
RAW264.7 cells were derived from BALB/c mice cells infected with Abelson leukemia virus which were considered to be a suitable model to study the function of macrophage because of their macrophage characteristics [3,24]
Summary
Polysaccharides, a class of natural macromolecule polymers which consist of more than 10 monosaccharides linked with glycosidic bonds, have a wide range of biological activities [1].Edible mushroom polysaccharides (EMPs) are a member of the polysaccharide family. Polysaccharides, a class of natural macromolecule polymers which consist of more than 10 monosaccharides linked with glycosidic bonds, have a wide range of biological activities [1]. EMPs have shown a wide range of biological functions, such as anti-tumor, anti-bacterial, antioxidant, antiviral, regulating gut microbiota and immunomodulatory activities [2,3,4]. Some edible mushrooms are involved in Chinese herbal medicine such as Ganoderma lucidum and Polyporus umbellatus. The mechanisms of Chinese herbal medicine polysaccharides are considered to participate in the activity regulation of T cells, B cells, macrophages, dendritic cells and natural killer (NK) cells, binding to specific receptors on the cell surface, activating various signal transduction pathways in the cell, stimulating cytokine or antibody secretion, and regulating the immune function of the body at multiple levels, multiple channels and multiple targets [6]. There have been many studies focusing on the immunomodulation of Nutrients 2019, 11, 1480; doi:10.3390/nu11071480 www.mdpi.com/journal/nutrients
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
