Abstract
BackgroundGolgi protein 73 (GP73) is a type II Golgi transmembrane protein. It is over-expressed in several cancers, including hepatocellular carcinomas, bile duct carcinomas, lung cancer and prostate cancer. However, there are few reports of GP73 in gastric cancer. This study is aimed at investigating the expression of GP73 and its relationship with clinical pathological characters in gastric cancer.MethodsGP73 mRNA level was determined by quantitative real-time RT-PCR in 41 pairs of matched gastric tumorous tissues and adjacent non-tumorous mucosal tissues. Western blotting was also performed to detect the GP73 protein level. GP73 protein expression was analyzed by immunohistochemistry in 52 clinically characterized gastric cancer patients and 10 non-tumorous gastric mucosal tissue controls.ResultsThe mRNA and protein level of GP73 were significantly down-regulated in gastric tumorous tissues compared with the non-tumorous mucosal tissues. In non-tumorous mucosa, strong diffuse cytoplasmic staining can be seen in cells located at the surface of the glandular and foveolar compartment; while in tumorous tissues, the staining was much weaker or even absent, and mainly in a semi-granular dot-like staining pattern. The expression level of GP73 protein was associated with patients’ gender and tumor differentiation.ConclusionsGP73 was normally expressed in non-tumorous gastric mucosa and down-regulated in gastric cancer. Its expression in gastric cancer was correlated with tumor differentiation.
Highlights
Golgi protein 73 (GP73) is a type II Golgi transmembrane protein
GP73 expression level in gastric tumor and non-tumorous tissue GP73 mRNA expression was significantly decreased in gastric tumor tissues compared to the adjacent nontumorous tissues (33/41; 80.5%)
The expression levels of GP73 relative to GAPDH were much lower in gastric tumorous tissues (0.259 ± 0.308) than that in non-tumorous mucosal tissues (0.584 ± 0.523; P
Summary
Golgi protein 73 (GP73) is a type II Golgi transmembrane protein. It is over-expressed in several cancers, including hepatocellular carcinomas, bile duct carcinomas, lung cancer and prostate cancer. This study is aimed at investigating the expression of GP73 and its relationship with clinical pathological characters in gastric cancer. GP73, known as GOLM1 and GOLPH2, is a type II Golgi transmembrane protein. The luminal C-terminal domain is the major function domain of GP73 [1,2,3]. A proproteinconvertase recognition site R52VRR55 is located within the C-terminal ectodomain; PC-mediated cleavage of GP73 transforms the intracellular, Golgi-localized GP73 to a soluble, secretory protein [6].
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