Abstract
Abstract Suppose that you are about to subculture a cell line: an activity common to all cell culture scientists, so familiar as to be completed almost without thinking. You clean your laminar flow cabinet, warm your bottle of fresh medium, locate your culture, and ensure that all your other equipment and materials are to hand. Then you disinfect your gloves, transfer one-fifth of the culture volume into a new flask, aseptically add four volumes of fresh medium, similarly add any other separate but essential medium components, label the new culture, and return this to the incubator. Finally you clean your cabinet, discard your contaminated materials, and return the medium and components to the fridge or freezer. Comfortable?
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