Abstract
Women infected with human papillomavirus (HPV) are at a higher risk of developing cervical lesions. In the current study, self and clinician-collected vaginal and cervical samples from women were processed to detect HPV DNA using polymerase chain reaction (PCR) with PGMY09/11 primers. HPV genotypes were determined using type-specific PCR. HPV DNA detection showed good concordance between self and clinician-collected samples (84.6%; kappa = 0.72). HPV infection was found in 30% women and genotyping was more concordant among high-risk HPV (HR-HPV) than low-risk HPV (HR-HPV). HPV16 was the most frequently detected among the HR-HPV types. LR-HPV was detected at a higher frequency in self-collected; however, HR-HPV types were more frequently identified in clinician-collected samples than in self-collected samples. HPV infections of multiple types were detected in 20.5% of clinician-collected samples and 15.5% of self-collected samples. In this study, we demonstrated that the HPV DNA detection rate in self-collected samples has good agreement with that of clinician-collected samples. Self-collected sampling, as a primary prevention strategy in countries with few resources, could be effective for identifying cases of HR-HPV, being more acceptable. The use of this method would enhance the coverage of screening programs for cervical cancer.
Highlights
Women infected with human papillomavirus (HPV) are at a higher risk of developing cervical lesions than those without HPV infection (Koutsky et al 1992)
We found a lower frequency of HPV infection in women ≥ 30 years (p = 0.009)
Six women tested HPV-positive on clinician-collected samples, but HPV-negative on self-collected samples
Summary
Women infected with human papillomavirus (HPV) are at a higher risk of developing cervical lesions than those without HPV infection (Koutsky et al 1992). The meta-analysis conducted in 2007 verified the accuracy of self and clinician-collected sampling to identify women with genital HPV infections and showed that the two methods had equivalent abilities to detect HPV DNA (Petignat et al 2007). The present study aimed to evaluate the HPV DNA detection and genotyping concordance between self and clinician-collected cervicovaginal and cervical samples.
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