Abstract
In order to avoid invasive sampling in pediatric patients, we examined the concentrations of LH and FSH in paired serum and urine samples from 43 children (age 0-16 yr) who had no interfering disorders or medication. Highly sensitive time-resolved immunofluorometric assay (IFMA) kits were obtained from Pharmacia-Wallac, Turku, Finland (LH and FSH Delfia®). The intraassay variation was <8%. The detection limit of the assay was 0.015 IU/L for LH and 0.018 IU/L for FSH. These sensitivity levels cover the whole prepubertal range of LH and FSH levels. Urinary gonadotropin levels were aimed to be corrected by the urinary density [concentration × (0,02/density-1)] and urinary creatinine levels (concentraiion/creatinine). The correlation between serum and urine gonadotropin values was good (see table). Besides good correlation, also the absolute concentrations were close to each other, which is not the case for growth hormone. Correction for urinary excretion did not improve the correlation:
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
