Abstract
Certain monkey pituitary preparations with low FSH biopotencies were found to contain unexpectedly large quantities of immunoreactive FSH when assayed by a specific radioimmunoassay for rhesus FSH. Exclusion chromatography of these and other preparations, and assay of the resulting fractions, suggested that rhesus pituitary FSH is heterogeneous, and that the ratio of immunological to biological activity is least for the largest FSH molecules. Further, the ratios of immunological to biological FSH activity of unfractionated pituitary extracts and sera from intact female monkeys were at least twice those of pituitary extracts and sera from ovariectomized animals. Exclusion chromatography revealed a preponderance of FSH components of larger molecular size in the extracts and sera from the ovariectomized animals, suggesting that differences in FSH structure may account for the observed discrepancies between activity ratios. That these differences were seen in sera as well as in pituitary extracts attests to t...
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