Gonadal development and associated changes in liver size and sexual steroids during the reproductive cycle of captive male and female Atlantic cod ( Gadus morhua L.)

Abstract

Gametogenesis in female and male Atlantic cod ( Gadus morhua L.) was investigated by sampling blood plasma and gonadal tissue from 19 to 33-month-old fish. The reproductive cycles of both female and male Atlantic cod are characterized by distinct annual variations in gonadal size and developmental stage and these are associated with changes in sex steroids and liver size. I H did not change during early gonadal development, but both spent females and males had lower I H than late maturing females and spermiating males, respectively. In females I G was correlated to plasma E2 levels and they were highest in spawning females. The lowest levels during the reproductive cycle were observed in spent females. Plasma T levels were low throughout ovarian development, and were at a minimum in spent females. 11-ketotestosterone in plasma of males increased rapidly during spermiation, while T increased at earlier testicular stages and reached maximum during spermiation. High plasma levels of steroids in male and female cod during spawning serve to promote further development and growth of less advanced stages of germ cells.