Abstract
Colonies of a new, stalked diatom species Gomphoneis tegelensis sp. nov. were sampled in September 2017 from Lake Tegel (Berlin, Germany) where this large species was first observed in 2009. Cultivation was attempted several times without success. A single-cell DNA extraction method was therefore tried to yield molecular and morphological data from non-cultured specimens. Single cells were selectively picked off their stalks and their cell walls broken to release the DNA, as PCR and sequencing of intact cells provided no results. To improve yield, the DNA of several cells was pooled after PCR. The remaining intact cells were used for ultrastructural studies under SEM. The closely related species Gomphoneis transylvanica is typified as a basis for proper taxon identification and for comparison with the newly described species. Gomphoneis tegelensis differs from G. transylvanica and Gomphoneis ohridana mainly in its striation pattern and the number of rows of areolae in each stria. Molecular data confirm that G. tegelensis belongs to the Cymbellales, but it does not belong to the Gomphonemataceae.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
