Abstract
Gliomas are the most common malignant tumors of the brain and are characteristic of severe migration and invasion. Glial cell line-derived neurotrophic factor (GDNF) promotes glioma development process. However, the regulatory mechanisms of promoting occurrence and development of glioma have not yet been clearly elucidated. In the present study, the mechanism by which GDNF promotes glioma cell migration and invasion through regulating the dispersion and location of the Golgi apparatus (GA) is described. Following GDNF treatment, a change in the volume and position of GA was observed. The stack area of the GA was enlarged and it was more concentrated near the nucleus. Golgin-160 and Golgi microtubule-associated protein 210 (GMAP210) were identified as target molecules regulating GA positioning. In the absence of either golgin-160 or GMAP210 using lentivirus, the migration and invasion of U251 cells were decreased, while it was increased following GDNF. It was also found that the GA was decreased in size and dispersed following golgin-160 or GMAP210 knockdown, as determined by GA green fluorescence assay. Once GDNF was added, the above phenomenon would be twisted, and the concentrated location and volume of the GA was restored. In combination, the present data suggested that the regulation of the position and size of the GA by golgin-160 and GMAP210 play an important role in U251 cell migration and invasion.
Highlights
Glioma is a heterogeneous, highly complicated central nervous system (CNS) tumor with an uncertain mechanism of initiation and progression[1], which results in an unfavorable outcome
The results showed that fragmented Golgi apparatus (GA) was reduced, and the GA concentrated and gathered near the nucleus in U251 cells following Glial cell line-derived neurotrophic factor (GDNF) treatment for different time points (Fig 1A)
The results showed that protein expression of golgin-160 and GMAP-210 were elevated in the GNDF treatment groups (Fig 2A), which was consistent with the increase in the stack area of the GA by Golgi-ID green staining (Fig 2C)
Summary
Highly complicated central nervous system (CNS) tumor with an uncertain mechanism of initiation and progression[1], which results in an unfavorable outcome. The invasion properties of glioblastoma render a radical surgery necessary and are responsible for its recurrence[2]. The migration and invasion of glioma cells. GDNF promotes U251 cells migration mediated by Golgi apparatus experimental guidance, Dian-Shuai Gao: Funding, project supervision, study conception and design
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