Gold-Polyoxoborates Nanocomposite Prohibits Adsorption of Bacteriophages on Inner Surfaces of Polypropylene Labware and Protects Samples from Bacterial and Yeast Infections.

Mateusz Wdowiak,Enkhlin Ochirbat,Jan Paczesny

doi:10.3390/v13071206

Mateusz Wdowiak, Enkhlin Ochirbat + Show 1 more

Open Access

https://doi.org/10.3390/v13071206

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Abstract

Bacteriophages (phages) are a specific type of viruses that infect bacteria. Because of growing antibiotic resistance among bacterial strains, phage-based therapies are becoming more and more attractive. The critical problem is the storage of bacteriophages. Recently, it was found that bacteriophages might adsorb on the surfaces of plastic containers, effectively decreasing the titer of phage suspensions. Here, we showed that a BOA nanocomposite (gold nanoparticles embedded in polyoxoborate matrix) deposited onto the inner walls of the containers stabilizes phage suspensions against uncontrolled adsorption and titer decrease. Additionally, BOA provides antibacterial and antifungal protection. The application of BOA assures safe and sterile means for the storage of bacteriophages.

Highlights

Bacteriophages, i.e., viruses that infect bacterial cells, were recognized early due to their potential application for medical purposes [1]
When the water wetting angle (WA) of the container insides is higher than a threshold, it is more favorable to “cover”
PFU/mLT4 virions from the suspension [13]. The magnitude of this effect varies for tubes purchased from various vendors and between batches provided by a single vendor

Summary

Introduction

Bacteriophages, i.e., viruses that infect bacterial cells, were recognized early due to their potential application for medical purposes [1]. Some phages are capable of retaining activity even after exposure to stress factors, such as high temperatures [8], pH [9], and organic solvents [10,11]. The resistance to external conditions is not the primary criterion for claiming phages’ value for medical purposes. More often, their virulence, selectivity, ease of manipulation, and/or modification are taken into account, despite phage stability being important. Phage stability determining factors were described in the review by Jończyk-Matysiak et al [12]. The authors focused on the effect of chemical substances, pH, temperature change (freezing, heat), UV-light, and methods of preparation and formulation

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