Abstract
Parkinson’s disease (PD) is a nervous disorder, affects physical movement, and leads to difficulty in balancing, walking, and coordination. A novel sensor is mandatory to determine PD and monitor the progress of the treatment. Neurofilament light chain (NfL) has been recognized as a good biomarker for PD and also helps to distinguish between PD and atypical PD syndromes. Immunosensor was generated by current–volt measurement on gap-fingered interdigitated electrode with silicon dioxide surface to determine NfL level. To enhance the detection, anti-NfL antibody was complexed with gold-nanourchin and immobilized on the sensing electrode. The current–volt response was gradually increased at the linear detection range from 100 fM to 1 nM. Limit of detection and sensitivity were 100 fM with the signal-to-noise ratio at n = 3 on a linear curve ( y = 0.081 x + 1.593; R 2 = 0.9983). Limit of quantification falls at 1 pM and high performance of the sensor was demonstrated by discriminating against other neurogenerative disease markers, in addition, it was reproducible even in serum-spiked samples. This method of detection system aids to measure the level of NfL and leads to determine the condition with PD.
Highlights
Parkinson’s disease (PD) is a progressive brain disorder, occurs in the area of the neurons or nerve cells, and causes problems in the movement.[1,2] A significant loss has been noticed with PD, caused uncomfortable lifestyle and severe health issues
To determine Neurofilament light chain (NfL) antigen, gold-nanourchin-antibody conjugation was followed as the probe on interdigitated electrode (IDE)
This immobilization process enhanced the numbers of anti-NfL antibody binding on the surface of IDE surface, which increases the chance for enhancing the NfL antigen detection
Summary
Parkinson’s disease (PD) is a progressive brain disorder, occurs in the area of the neurons or nerve cells, and causes problems in the movement.[1,2] A significant loss has been noticed with PD, caused uncomfortable lifestyle and severe health issues. Identifying and diagnosing PD with an ideal biomarker is mandatory to give the proper followup treatment.[3] Neurofilament light chain (NfL) is a neuronal cytoplasmic protein, expressed highly in the area of myelinated axons. Nanomaterials and Nanotechnology neurogenerative, and PD.[4,5,6] In particular, it has been proved that the level of NfL is increasing in PD disorder patient and potential alternate biomarkers for neurogenerative diseases. Generating a detection system to quantify NfL level in the cerebrospinal fluid helps to diagnose PD.[7,8] Predominantly, the surface of the sensor plays a crucial role for high-performance detection with a rightly oriented biomolecular immobilization
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