Abstract
We explore the feasibility of using gold nanorods with efficient two-photon luminescence properties as contrast agents for intravital imaging of neoplasia. This investigation spanned ex vivo characterization in cells/tissue to in vivo implementation in an oral carcinogenesis model. GNRs were >40 times brighter than surrounding tissue. Intravital imaging revealed 3D microvasculature, and in dysplasia, abnormal vessels (dense and tortuous) compared to normal. GNRs were diffusely distributed in lesions after 24 hours. No known previous study has revealed abnormal vessel structure in dysplasia by imaging. Results suggest GNRs can function as high-contrast agents for in vivo visualization of carcinogenesis features.
Highlights
Efficient nanoparticles exhibiting two-photon induced photoluminescence are of great interest as contrast agents for in vivo optical imaging, including molecular imaging of cancer, due to their ability to be excited with extremely low incident powers, their well-defined spectral properties, and their potential for enabling large imaging depths using intravital twophoton microscopy [1,2]
We explore the feasibility of using gold nanorods with efficient two-photon luminescence properties as contrast agents for intravital imaging of neoplasia
Samples labeled with Gold nanorods (GNRs) were illuminated with incident powers as low as 0.2 mW, which resulted in an average signal-to
Summary
Efficient nanoparticles exhibiting two-photon induced photoluminescence are of great interest as contrast agents for in vivo optical imaging, including molecular imaging of cancer, due to their ability to be excited with extremely low incident powers, their well-defined spectral properties, and their potential for enabling large imaging depths using intravital twophoton microscopy [1,2]. Previous studies demonstrating the potential benefits of GNRs as high contrast TPL agents for cancer imaging have been reported for studies performed in cell cultures or tissue phantoms [4,8]. Gold nanoshells have been imaged in vivo and though one study found their properties to be comparable to GNRs, only nanoshells were delivered in vivo [2]. No known reports have demonstrated the potential use of GNRs as contrast agents for intravital multi-photon imaging in neoplasia
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
