Abstract
In this study, gold nanoparticles and thermochromic composite films modified screen-printed carbon electrodes (TM-AuNPsSPCEs) were developed as a platform for the simultaneous detection of protein and temperature. The TM-AuNPs composited film had better sensitivity resulting from the gold nanoparticles amplification effect. A phase transition model analysis of TM-AuNPs films found that the TM-AuNPs films had three-phase transition intervals (80℃) which accommodated the temperature requirements for protein denaturation. When used to detect different concentrations of haemoglobin (Hb) solution, the TM-AuNPs modified SPCEs had a better sensitivity in detecting the different concentrations in comparison to TM and AuNP modified SPCEs which showed no clear sensitivity towards the different Hb concentrations. The dual detection and excellent sensitivity show a good application prospect for the study of the TM-AuNPs composite film.
Highlights
Gold nanoparticles (AuNPs) have been widely applied in diagnostics, therapy, prophylaxis, and hygiene since the discovery of immunogold labelling by Faulk and Taylor in the 1970s [1]
When used to detect different concentrations of haemoglobin (Hb) solution, the thermochromic material (TM)-AuNPs modified SPCEs had a better sensitivity in detecting the different concentrations in comparison to TM and AuNP modified SPCEs which showed no clear sensitivity towards the different Hb concentrations
A thin layer of AuNPs with negative charge was electrodeposited on the surface of SPCE substrate and a thin TM film with positive charge was immobilized on the surface of AuNPs via electrostatic interaction Figure 1(a)
Summary
Gold nanoparticles (AuNPs) have been widely applied in diagnostics, therapy, prophylaxis, and hygiene since the discovery of immunogold labelling by Faulk and Taylor in the 1970s [1]. Narhi et al [11] found that increasing protein concentration increased the rate of protein aggregation during thermal treatment This effect shows that the quantitative analysis of factors affecting protein aggregation such as detecting protein concentration at different temperatures and time is very important. The surface of screen-printed electrodes was modified with gold nanoparticles and thermochromic composite film for the sensing of protein and temperature in real-time. Gold nanoparticles (AuNPs) and thermochromic materials have both attracted considerable attention owing to their wide applications [17] [18] [19]. Owing to gold nanoparticles amplification effect between TM and SPCEs, enhanced voltammetric peaks at the TM-AuNPsSPCEs. the proposed electrode was applied for the simultaneous determination of protein and temperature
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