Abstract
Abstract A novel electrochemical sensor based on the immobilization of tyrosinase (tyr) onto gold nanoparticles (nano-Au) and thioctic acid amide (T-NH 2 ) self-assembled monolayers (SAMs)-modified gold electrode has been developed for the determination of bisphenol A (BPA). It was found that the nano-Au could significantly enhance the electrochemical response of tyr/nano-Au/T-NH 2 /Au electrode to BPA, and the enhancement effect of nano-Au on the current response was also related to the enzyme. The results indicated that the biosensor could be used as a detector for BPA determination with a linear range from 3.99 × 10 −7 mol/L to 2.34 × 10 −4 mol/L and a detection limit of 1.33 × 10 −7 mol/L. In addition, this biosensor showed good reproducibility.
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