Abstract
Enteric bacterial human pathogens, i.e., Escherichia coli, Staphylococcus aureus, Bacillus subtilis and Klebsiella pneumoniae, are the major cause of diarrheal infections in children and adults. Their structure badly affects the human immune system. It is important to explore new antibacterial agents instead of antibiotics for treatment. This project is an attempt to explain how gold nanoparticles affect these bacteria. We investigated the important role of the mean particle size, and the inhibition of a bacterium is dose-dependent. Ultra Violet (UV)-visible spectroscopy revealed the size of chemically synthesized gold nanoparticle as 6–40 nm. Atomic force microscopy (AFM) analysis confirmed the size and X-ray diffractometry (XRD) analysis determined the polycrystalline nature of gold nanoparticles. The present findings explained how gold nanoparticles lyse Gram-negative and Gram-positive bacteria.
Highlights
In recent years, nanoparticles (NPs) have become a promising candidate in place of conventional materials with enormous applications in the fields of science and engineering
The main advantage of gold NPs is that they are easy to synthesize by chemical reduction technique and they have a low toxicity compared to various nanomaterials
Escherichia coli, Klebsiella pneumonia, Staphylococcus aureus, and Bacillus subtilis were provided by the Microbiology Department, University of Veterinary and Animal Sciences, Lahore
Summary
Nanoparticles (NPs) have become a promising candidate in place of conventional materials with enormous applications in the fields of science and engineering. The uniqueness of NPs is due to the higher surface-to-volume ratio and the increased number of atoms at their grain boundaries [1] They proved to be significant materials in the advancement of various novel devices that are used in different biological, physical, pharmaceutical and biomedical applications [1,2,3]. The main achievement of the current research is fabricating gold NPs of a small size by the reduction of NaBH4 instead of the familiar Turkevich method Different doses of these NPs were utilized to check the antibacterial mechanism against four human pathogen bacteria: E. coli, S. aureus, B. subtilis and Klebsiella pneumonia. From these doses, the zones of inhibition were studied by the
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