Gold Nanoparticle-Enhanced and Roll-to-Roll Nanoimprinted LSPR Platform for Detecting Interleukin-10.

Seung Hee Baek,Sunwoong Lee,Seonki Hong,Yeo Hyang Kim,Jong G Ok,Hyun Woo Song,Jun Seok Park,Jung-Eun Kim,Jung-Sub Wi,Hye Jin Lee,Sung-Wook Nam,Moon Kyu Kwak

doi:10.3389/fchem.2020.00285

Abstract

Localized surface plasmon resonance (LSPR) is a powerful platform for detecting biomolecules including proteins, nucleotides, and vesicles. Here, we report a colloidal gold (Au) nanoparticle-based assay that enhances the LSPR signal of nanoimprinted Au strips. The binding of the colloidal Au nanoparticle on the Au strip causes a red-shift of the LSPR extinction peak, enabling the detection of interleukin-10 (IL-10) cytokine. For LSPR sensor fabrication, we employed a roll-to-roll nanoimprinting process to create nanograting structures on polyethylene terephthalate (PET) film. By the angled deposition of Au on the PET film, we demonstrated a double-bent Au structure with a strong LSPR extinction peak at ~760 nm. Using the Au LSPR sensor, we developed an enzyme-linked immunosorbent assay (ELISA) protocol by forming a sandwich structure of IL-10 capture antibody/IL-10/IL-10 detection antibody. To enhance the LSPR signal, we introduced colloidal Au nanocube (AuNC) to be cross-linked with IL-10 detection antibody for immunogold assay. Using IL-10 as a model protein, we successfully achieved nanomolar sensitivity. We confirmed that the shift of the extinction peak was improved by 450% due to plasmon coupling between AuNC and Au strip. We expect that the AuNC-assisted LSPR sensor platform can be utilized as a diagnostic tool by providing convenient and fast detection of the LSPR signal.

Highlights

To improve the Localized surface plasmon resonance (LSPR) detection capability, we developed dual plasmonic structures by inducing plasmon coupling between colloidal Au nanocube (AuNC) and multi-bent Au nanostrip
We wrapped a cylindrical roll with an imprinting mold for stamping nanoarchitecture on the polyethylene terephthalate (PET) film coated with UV-curable polymer resin using an airbrush coating process (Koo et al, 2016)
Using AuNC and Au LSPR strip, we successfully demonstrated the LSPRbased analytical strategy for IL-10 detection

Summary

Introduction

Localized surface plasmon resonance (LSPR) sensors can monitor a variety of binding events including protein-ligand interactions (McFarland and Van Duyne, 2003; Holzinger et al, 2014; Jang et al, 2014; Liu et al, 2018), DNA hybridizations (Storhoff et al, 2004; Sönnichsen et al, 2005; Baek et al, 2014), and biomarker interactions (Im et al, 2014; Xu et al, 2018; Bellassai et al, 2019; Wang et al, 2019). Dual Au LSPR Assay Platform shift of the extinction peak, which is measured by ultraviolet-visible spectroscopy (UV-vis) (Sepúlveda et al, 2009). It is known that the extinction peak shift stems from the changes of the refractive index on the surface of the LSPR structure when analyte binds with the plasmonic nanostructures such as gold (Au) and silver (Ag) (Willet and Van Duyne, 2007; Hall et al, 2011). To improve the LSPR detection capability, we developed dual plasmonic structures by inducing plasmon coupling between colloidal Au nanocube (AuNC) and multi-bent Au nanostrip

Methods

Results

Conclusion