Abstract
Detection of host integrated viral oncogenes are critical for early and point-of-care molecular diagnostics of virus-induced carcinoma. However, available diagnostic approaches are incapable of combining both cost-efficient medical diagnosis and high analytical performances. To circumvent this, we have developed an improved IDE-based nanobiosensor for biorecognition of HPV-16 infected cervical cancer cells through electrochemical impedance spectroscopy. The system is fabricated by coating gold (Au) doped zinc oxide (ZnO) nanorods interfaced with HPV-16 viral DNA bioreceptors on top of the Interdigitated Electrode (IDE) chips surface. Due to the concurrently improved sensitivity and biocompatibility of the designed nanohybrid film, Au decorated ZnO-Nanorod biosensors demonstrate exceptional detection of HPV-16 E6 oncogene, the cancer biomarker for HPV infected cervical cancers. This sensor displayed high levels of sensitivity by detecting as low as 1fM of viral E6 gene target. The sensor also exhibited a stable functional life span of more than 5 weeks, good reproducibility and high discriminatory properties against HPV-16. Sensor current responses are obtained from cultured cervical cancer cells which are close to clinical cancer samples. Hence, the developed sensor is an adaptable tool with high potential for clinical diagnosis especially useful for economically challenged countries/regions.
Highlights
Human Papillomavirus (HPV), is the prime causative agent in the development of cervical cancers predominantly occurring within underdeveloped, third world countries
We have described the use of interdigitated electrode (IDE) sensor platforms fabricated with an aptitude for sensitive and specific detection of HPV subtype-16 (HPV-16) infected cervical cells
Our biosensor system utilizes short, thiolated-probe sequences complementary to the E6 oncogene as the biorecognition element or capture probe of HPV-16 viruses since it is known that HPV-16 closely associates with cervical epithelial tissue through genomic integration of E6 virulent genes
Summary
Human Papillomavirus (HPV), is the prime causative agent in the development of cervical cancers predominantly occurring within underdeveloped, third world countries. Before applying the designed sensor platform for the detection of HPV-16 E6 oncogene in cervical cancer gDNA samples, the performance of the designed E6 capture probe was tested through PCR analysis to determine whether the in-sillico confirmed target binding properties of the probe performs well under realistic and practical conditions.
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