Abstract
Herein, an Au@Pt nanozyme-mediated magnetic relaxation switching (MRS) DNA biosensor has been developed for the rapid detection of Listeria monocytogenes. Au@Pt nanoparticle was with enhanced stability and high peroxidase activity for decomposing the hydrogen peroxide. After DNA hybridization and magnetic separation, the conjugates of MNP180-probe1-L. monocytogenes DNA-probe2-Au@Pt could adjust the hydrogen peroxide-mediated Mn(VII)/Mn(II) conversion, and thus leading to the change of the transverse relaxation time. The amount of Au@Pt was directly proportional to the concentration of L. monocytogenes. The Au@Pt nanozyme and the hydrogen peroxide-mediated Mn(VII)/Mn(II) signal conversion system contributed to the sensitivity and stability of this sensor. This sensor enabled the detection of L. monocytogenes with a LOD of 30 CFU/mL, which was 33-folds enhancement compared with that of enzyme-linked immunosorbent assay, providing a promising platform to trace the foodborne pathogen in the complex food matrix.
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