Abstract
β-Type platelet-derived growth factor receptor (PDGFRB) is a typical tyrosine kinase, as a candidate gene associated with reproduction. Its main roles include regulation of gonocytes (migration and proliferation) and of the cell cycle. The objectives of this study were to identify mRNA expression of the goat PDGFRB gene, as well as insertion/deletion (indel) variants and their association with litter size in 1122 healthy Shaanbei white cashmere goats. The results revealed that PDGFRB was widely expressed in all tested tissues, and the expression levels in testes at different developmental stages indicated a potential association with the mitosis-to-meiosis transition. Furthermore, the expression of PDGFRB was relatively higher in the ovary tissue of mothers of two lambs compared with mothers of single lamb. These results implied that PDGFRB was related to goat fertility. Meanwhile, two intronic indels, 5 bp (n = 501) and 10 bp (n = 1122), were identified. Statistical analysis revealed that only the 10 bp indel was associated with first-born litter size (n = 1122, p = 6.030 × 10−5), and that individuals of the genotype insertion/deletion had larger litter sizes than those of genotype insertion/insertion. Overall, these results indicated that the 10 bp indel of PDGFRB could be used in marker-assisted selection during goat genetic breeding.
Highlights
Since the beginning of the twenty-first century, the goat industry in China has developed rapidly and its share in the national economy has increased year by year
The results revealed that PDGFRB was widely expressed in all tested tissues, and the expression levels in testes at different developmental stages indicated a potential association with the mitosis-to-meiosis transition
In 2017, using genome-wide association study (GWAS), Liu et al [8] and Qin et al [9] found that a single-nucleotide polymorphism (SNP) of the b-type platelet-derived growth factor receptor (PDGFRB) gene was significantly associated with semen production traits in Chinese Holstein bulls, which has since been validated by a larger study
Summary
Since the beginning of the twenty-first century, the goat industry in China has developed rapidly and its share in the national economy has increased year by year. In 2017, using GWAS, Liu et al [8] and Qin et al [9] found that a single-nucleotide polymorphism (SNP) of the b-type platelet-derived growth factor receptor (PDGFRB) gene was significantly associated with semen production traits in Chinese Holstein bulls, which has since been validated by a larger study. These results implied the feasibility of screening crucial genetic variations by combining different methods
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