Abstract
The GO-SHIP nutrient manual covers all aspects of nutrient analysis from basic sample collection and storage, specifically for Continuous Flow analysis using an Auto-Analyzer, and describes some specific nutrient methods for Nitrate, Nitrite, Silicate, Phosphate and Ammonium that are in use by many laboratories carrying out at-sea analysis and repeat hydrography sections across the world. The focus is on segmented flow analyzers not flow injection analyzers. It also covers laboratory best practices including quality control and quality assurance (QC/QA) procedures to obtain the best results, and suggests protocols for the use of reference materials (RM) and certified reference materials (CRMs).
Highlights
The availability of inorganic macronutrients {nitrate (NO3), phosphate (PO4), silicic acid [Si(OH)4] commonly referred to as “silicate,” ammonium (NH4), and nitrite (NO2)} in upper ocean waters frequently limits and regulates the amount of organic carbon fixed by phytoplankton, thereby constituting a key control mechanism of carbon and biogeochemical cycling
Internal consistency of nutrient data in the order of 1–3% has been achieved through secondary quality control (QC) procedures implemented in the GLODAP and CARINA Projects (Tanhua et al, 2010)
A clean sample can be collected with bare hands without the use of gloves, powder free vinyl gloves are highly recommended for use in the lab and for sample collection at sea
Summary
The availability of inorganic macronutrients {nitrate (NO3), phosphate (PO4), silicic acid [Si(OH)4] commonly referred to as “silicate,” ammonium (NH4), and nitrite (NO2)} in upper ocean waters frequently limits and regulates the amount of organic carbon fixed by phytoplankton, thereby constituting a key control mechanism of carbon and biogeochemical cycling. The optical effect caused by mixing two solutions of different densities, such as ultrapure wash water with a seawater sample, is called the Schlieren effect This effect is greatly reduced in modern analyzers by flowcells and detectors that allow the inter-sample bubble to pass through them. The use of an internal standard was further developed at NIOZ Their procedure calls for preparing a sufficient quantity of mixed concentrated nutrient standard in ultrapure water, which is preserved by the addition of mercuric chloride. It is prepared independently of the primary and working standards that are used to calibrate the individual analysis runs. If reference materials were analyzed, the manufacturer, batch number, and given values should be included with the bottle file
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