Abstract
The present study was undertaken to investigate the mRNA expression for gonadotropin-releasing hormone (GnRH) receptor in bovine ovary. Granulosa cells from small (< 4 mM), medium (5–8 mM) and large follicles (> 8 mM) and tissues from corpora lutea (CL) of different stages: Stage I (days 1–4), Stage II (days 5–10), Stage III (days 11–17), and Stage IV (days 18–21, days after ovulation) were harvested from bovine ovaries collected at a local abattoir. The mRNA isolated from representative samples was subjected to reverse transcription-polymerase chain reaction (RT-PCR) using gene sequence specific primers. The resultant PCR amplified gonadotropin-releasing horm one receptor (GnRH-R) cDNA products were identified and confirmed through Southern blot hybridization and nucleotide sequence analysis, respectively. The results showed the presence of GnRH-R mRNA transcripts in both follicles and CL. Key words: Bovine, granulosa cells, corpus luteum, GnRH receptor, mRNA
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