Gnotobiotic, SPFならびにConventional ICRマウスの糞便菌そうの検索

Abstract

Fecal flora was examined in six groups of ICR mice which were reared in bacteriologically different environments. As shown in Table 1, gnotobiotic mice (Groups I & II) harbored only enteric bacteria, streptococci, staphylococci and lactobacilli. Bacteroides and yeasts appeared in two groups of SPF mice (Groups III & IV) which were derived from the mice of Group I. SPF mice of Group V, which were kept in an SPF room for four years, and conventional mice (Group VI), which were derived from the mice of Group V, harbored enteric bacteria, streptococci, staphylococci, lactobacilli, bacteroides, yeasts, aerobic spore formers, clostridia and pseudomonads.Subsequently, the organisms grown on DHL, SF, mannitol-salt and LBS agar plates were divided into several genera, species or biotypes (Table 2) . The organisms on DHL agar plates from gnotobiotic mice showed homologous properties and were classified in genus Proteus. On the other hand, the organisms consisting of Proteus and Klebsiella were isolated from SPF mice of Groups III and IV. SPF mice of Group V harbored Citrobacter, Escherichia and Klebsiella, while conventional mice harbored Escherichia including hydrogen-sulphide producing E. coli, Proteus and Citrobacter. As can be seen from Table 2, the same results as above were observed using SF, mannitol-salt and LBS agar plates, i. e, the number of species or types harbored increased when the gnotobiotic mice were transferred from isolator to SPF room or the SPF mice from SPF room to conventional room.