Günlük Bıldırcın Civcivlerinde Maternal Hipoksiyanın Uyardığı Çift Yönlü Morfolojik Özelliklerin Gelişme Stabilitesi ve Civciv Gelişimi Üzerine İn Ovo Enjeksiyon Aracılığı ile Antioksidanların Etkileri

Abstract

The study aimed to investigate effects of antioxidants via in ovo (IO) injection, one of the applications based on maternal effects, to different sacs of embryonated eggs obtained from quail breeders laid at high altitude on chick development and developmental stability of bilateral morphological characteristics. A total of 180 incubating eggs obtained for 7 days from 40 females at 120 days-old at a 1720 m-altitude were randomly divided into 3 groups as control (CONT), IO alpha tocopherol ( α-TO ) and IO ascorbic acid (AA). Sixty eggs of the control was non- i njection. The rest of 120 eggs (60 eggs/group) assigned to injection groups were injected into yolk sac a 25 µl vitamin E solution containing 3.75 mg α-TO by 120 th hour of incubation, and amniotic sac a 25 μl solution containing 2.5 mg AA by 360 th hour of incubation. At hatch, chick weight, organ weights, and chick length, bilaterally lengths of face, middle toe and shank were measured. Relative asymmetry (RA) of these traits were calculated. The effect of IO administration is insignificant on the lengths of chick, face and middle toe and weights of chick, and relative liver, heart and brain. Whereas the s hank length increased in the AA group, it decreased in the α-TO group. The RA of middle toe length was higher in the AA group and was lower in the α-TO group than CONT. The RA shank length was found the highest in the AA group differently from other groups. The mean RA of examined l engths was the highest i n the AA group and was the lowest in the α-TO group. The yolk sac weight was significantly lower in the CONT and α-TO groups than AA group. Proventriculus + gizzard weight was the lowest in the AA group and was the highest in the α-TO group. Injected antioxidants to embryonic sacs as a tool to eliminate the adverse effects of hypoxic-stress on several developmental traits and developmental stabiliy of bilateral morphological traits can be resulted in antioxidant-specific in day-old quail chicks.