GmPRP2 promoter drives root-preferential expression in transgenic Arabidopsis and soybean hairy roots.

Li Chen,Tianfu Han,Shi Sun,Wensheng Hou,Bingjun Jiang,Cunxiang Wu

doi:10.1186/s12870-014-0245-z

Li Chen, Tianfu Han + Show 4 more

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https://doi.org/10.1186/s12870-014-0245-z

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Abstract

BackgroundPromoters play important roles in gene expression and function. There are three basic types of promoters: constitutive, specific, and inducible. Constitutive promoters are widely used in genetic engineering, but these promoters have limitations. Inducible promoters are activated by specific inducers. Tissue-specific promoters are a type of specific promoters that drive gene expression in specific tissues or organs. Here, we cloned and characterized the GmPRP2 promoter from soybean. The expression pattern indicated that this promoter is root-preferential in transgenic Arabidopsis and the hairy roots of soybean. It can be used to improve the root resistance or tolerance to pathogens, pests, malnutrition and other abiotic stresses which cause extensive annual losses in soybean production.ResultsThe GmPRP2 promoter (GmPRP2p-1062) was isolated from soybean cv. Williams 82. Sequence analysis revealed that this promoter contains many cis-acting elements, including root-specific motifs. The GmPRP2p-1062 and its 5’-deletion fragments were fused with the GUS reporter gene and introduced into Arabidopsis and the hairy roots of soybean to further determine promoter activity. Histochemical analysis in transgenic Arabidopsis showed that GUS activity was mainly detected in roots and hypocotyls in all deletion fragments except GmPRP2p-471 (a 5’-deletion fragment of GmPRP2p-1062 with 471 bp length). GUS activity was higher in transgenic Arabidopsis and hairy roots with GmPRP2p-1062 and GmPRP2p-852 (a 5’-deletion fragment of GmPRP2p-1062 with 852 bp length) constructs than the other two constructs. GUS activity was enhanced by NaCl, PEG, IAA and JM treatments and decreased by SA, ABA and GA treatments in transgenic Arabidopsis.ConclusionsGmPRP2p-1062 is a root-preferential promoter, and its core fragment for root-preferential expression might lie between −369 and +1. GmPRP2p-852 may be useful in the genetic engineering of novel soybean cultivars in the future.Electronic supplementary materialThe online version of this article (doi:10.1186/s12870-014-0245-z) contains supplementary material, which is available to authorized users.

Highlights

Promoters play important roles in gene expression and function
Detection of GmPRP2 expression by quantitative realtime PCR GmPRP2 expression was investigated by real-time PCR from root, stem, leaf, flower, seed and hypocotyl
Several putative cis-regulatory elements were deciphered from the promoter sequence of GmPRP2 (Figure 2A)

Summary

Introduction

Promoters play important roles in gene expression and function. There are three basic types of promoters: constitutive, specific, and inducible. Promoters play a very important role in the initiation and regulation of gene transcription, and they are important components in transgenic engineering [1]. Promoters can be divided into three types: constitutive, specific, and inducible. Constitutive promoters are widely used in genetic engineering. The Inducible promoters are often regulated by particular chemical and physical factors, such as light, wounding, temperature, pH, hormones [6]. They can strongly enhance gene expression, and some of them are specific [7]

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