GmCYP82A3, a Soybean Cytochrome P450 Family Gene Involved in the Jasmonic Acid and Ethylene Signaling Pathway, Enhances Plant Resistance to Biotic and Abiotic Stresses.

Qiang Yan,Shuping Gan,Han Xing,Xiaoxia Cui,Daolong Dou,Shuai Lin,Hon-Ming Lam

doi:10.1371/journal.pone.0162253

Abstract

The cytochrome P450 monooxygenases (P450s) represent a large and important enzyme superfamily in plants. They catalyze numerous monooxygenation/hydroxylation reactions in biochemical pathways, P450s are involved in a variety of metabolic pathways and participate in the homeostasis of phytohormones. The CYP82 family genes specifically reside in dicots and are usually induced by distinct environmental stresses. However, their functions are largely unknown, especially in soybean (Glycine max L.). Here, we report the function of GmCYP82A3, a gene from soybean CYP82 family. Its expression was induced by Phytophthora sojae infection, salinity and drought stresses, and treatment with methyl jasmonate (MeJA) or ethephon (ETH). Its expression levels were consistently high in resistant cultivars. Transgenic Nicotiana benthamiana plants overexpressing GmCYP82A3 exhibited strong resistance to Botrytis cinerea and Phytophthora parasitica, and enhanced tolerance to salinity and drought stresses. Furthermore, transgenic plants were less sensitive to jasmonic acid (JA), and the enhanced resistance was accompanied with increased expression of the JA/ET signaling pathway-related genes.

Highlights

Soybean (Glycine max L.) is an agronomic crop grown throughout the world
In Sloan, GmCYP82A3 was up-regulated after P. sojae infection, and reached the highest level at 12 hpi
We performed a functional analysis of the soybean P450 monooxygenases (P450s) gene, called GmCYP82A3, which is highly expressed in partial resistant soybean cultivars during P. sojae infection and underlying the quantitative trait loci (QTL) conferring resistance to P. sojae [14]

Summary

Introduction

Soybean (Glycine max L.) is an agronomic crop grown throughout the world. It is an important source of vegetable protein and oil for humans and animals, but it is a source of biofuels. Soybean seeds contain a number of high-value secondary compounds with nutraceutical properties such as isoflavones, saponins, and tocopherols [1,2,3]. Phytophthora root and stem rot caused by Phytophthora sojae is one of the most destructive soybean diseases, results in annual losses of $200 million in the USA and $1–2 billion globally [4]. The effective way to protect soybean against P. sojae infection is breeding with dominant Rps (Resistance to Phytophthora sojae) genes. Continuous utilization of a single Rps gene may PLOS ONE | DOI:10.1371/journal.pone.0162253. Continuous utilization of a single Rps gene may PLOS ONE | DOI:10.1371/journal.pone.0162253 September 2, 2016

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Results

Conclusion