Abstract
Soybean bacterial disease is a worldwide disease that can cause yield and quality decline. Although the recognition of soybean bacterial diseases has been reported in detail, but for the identification of resistant and susceptible cultivars, different researchers have different methods and standards. To detect the pathogenic effect of bacterial diseases, we first separated bacterial strains from soybean field. Finally, eight strains of soybean bacterial diseases were isolated and identified. By the screening the sequence of the bacterial 16S rDNA, seven of eight strains were identified belong to Pseudomonas, another strain belong to Xanthomonas. In order to study the relevant pathogen of soybean defense response, we used the isolated pathogen strain to inoculate the resistant cultivar Hefeng35 and sensitive cultivar Suinong14. Then the genes might involve in regulate soybean resistant to bacterial disease were analyzed, including GmBAK1 and GmFLS2. FLS2 homolog gene were Gm05g25830, Gm08g08810; BAK1 homolog gene were Gm15g05730, Gm08g07930, Gm10g36280, Gm05g24790, Gm20g31320, Gm05g24770, Gm08g19270, and Gm02g08360. By the qRT-PCR analysis we detected the gene expression pattern response to pathogen, we found the expression of Gm05g24770, Gm05g24790, and Glyma05g25830 showed significant different between Suinong14 and Hefeng35. We illustrated the GmBAK1 and GmFLS2 transcription level against bacterial speck disease pathogen infection and revealed preliminary the major gene, providing a reference direction for further research.
Highlights
Soybean originated in China, and spread around the world with the circulation of commodity trade
Through the sampling in three places, it was found that soybean bacterial disease was common in Heilongjiang Province
In the resistant variety Hefeng35, BAK1 gene can be expressed in large quantity in the shortest time of 0.5h, and peak value in the latest time of 3 hours; in the susceptible variety Suinong14, the expression of BAK1 gene is not obvious in Hefeng35, and the expression quantity is basically unchanged or decreased
Summary
Soybean originated in China, and spread around the world with the circulation of commodity trade. In the United States, soybean yield reduction caused by bacterial diseases can reach 4-40% [8,9,10,11]. It can be divided into three kinds: soybean bacterial spot, Pseudomonas syringae pv. Foreign countries are mainly distributed in Japan, Korean Peninsula, Australia, the United States, New Zealand and other countries. It is one of the major diseases in soybean production areas in the world, which can infect soybean, lunar bean, bean and other legume plants
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