Abstract
Abstract Glyphosate, a systemic herbicide, is used against weeds in agricultural fields as well as against invasive plants in pastures, forest plantations, and urban environments. Its frequent and widespread use can negatively impact the surrounding natural non-target vegetation following the accidental drift of spray droplets, leaching, or persistence as residues in the soil. Because ferns possess a life cycle with independent sporophytic and gametophytic generations, herbicides may cause a different impact on each life stage. The objective of the present study was to evaluate the effect of four concentrations of glyphosate (0.33, 0.65, 2.72, and 10.89 g active ingredient L-1) and water as control treatments on spores, gametophytes, and two sporophyte size classes of one native (Blechnum appendiculatum Willd.) and two introduced fern species in Mexico (Macrothelypteris torresiana (Gaudich.) Ching and Thelypteris dentata (Forssk.) E.P. St.John). Spore germination was evaluated 10 days after herbicide trea...
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