Abstract
BackgroundHigh mobility group box 1 (HMGB1) plays important roles in a large variety of diseases; glycyrrhizin (GL) is recognized as an HMGB1 inhibitor. However, few studies have focused on whether glycyrrhizin can potentially improve the outcome of traumatic pancreatitis (TP) by inhibiting HMGB1.MethodsA total of 60 male Wistar rats were randomly divided into three groups (n = 20 in each): Control group, TP group and TP-GL group. Pancreatic trauma was established with a custom-made biological impact machine-III, and GL was administered at 15 minutes after the accomplishment of operation. To determine survival rates during the first 7 days after injury, another 60 rats (n = 20 in each) were grouped and treated as mentioned above. At 24 hours of induction of TP, the histopathological changes in pancreas were evaluated and serum amylase levels were tested. Serum tumor necrosis factor α (TNF-α), interleukin 6 (IL-6), and HMGB1 were measured using enzyme linked immunosorbent assay. HMGB1 expressions in pancreas were measured using immunohistochemical staining, Western blot and Real-Time PCR analysis.ResultsSerum levels of HMGB1, TNF-α and IL-6 were increased dramatically in TP group at 24 hours after induction of TP. However, these indicators were reduced significantly by GL administration in TP-GL group comparing with TP group (P<0.05). Meanwhile, survival analysis showed that the seven-day survival rate in TP-GL group was significantly higher than that in TP group (85% versus 65%, P<0.05). GL treatment significantly decreased the pancreatic protein and mRNA expressions of HMGB1 and ameliorated the pancreatic injury in rats with TP.ConclusionsGlycyrrhizin might play an important role in improving survival rates and ameliorating pancreatic injury of TP by suppression of the expressions of HMGB1 and other proinflammatory cytokine.
Highlights
Pancreatic trauma is rare, occurring in only 2% to 5% of trauma victims, it is often imperceptible and intractable with a higher morbidity and mortality
Glycyrrhizin might play an important role in improving survival rates and ameliorating pancreatic injury of traumatic pancreatitis (TP) by suppression of the expressions of High mobility group box 1 (HMGB1) and other proinflammatory cytokine
We have developed an experimental model of isolated traumatic pancreatitis [15] in rats and have been interested in the mechanisms and therapies of traumatic pancreatitis [16]
Summary
Pancreatic trauma is rare, occurring in only 2% to 5% of trauma victims, it is often imperceptible and intractable with a higher morbidity and mortality. Most pancreatic injuries in China are due to blunt abdominal trauma, such as motor vehicle crashes, falls, bicycle handlebar injuries, etc., while in Western countries, pancreatic injuries are due to penetrating abdominal trauma. To determine survival rates during the first 7 days after injury, another 60 rats (n520 in each) were grouped and treated as mentioned above. Results: Serum levels of HMGB1, TNF-a and IL-6 were increased dramatically in TP group at 24 hours after induction of TP. These indicators were reduced significantly by GL administration in TP-GL group comparing with TP group (P,0.05). GL treatment significantly decreased the pancreatic protein and mRNA expressions of HMGB1 and ameliorated the pancreatic injury in rats with TP
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