Glycyrrhizic acid (GA) from Taverniera cuneifolia (Roth) Arn.: In vitro germination and somatic embryogenesis via tissue culture studies

Abstract

Glycyrrhizic acid (GA) is commercially extracted from plant Glycyrrhiza glabra L., while Taverniera cuneifolia (Roth) Arn, licorice of India comprise similar phytochemical constituents but has been least studies on commercial applications. In 21st century, scientist and pharmacognocist explored new biomolecules in several plants which proved to comprise medicinal properties for examination against bacteria and fungi, to circumvent loss of native plants, for better and quantitative extraction using plants of similar properties. GA from the root extracts of plants exhibits promising anti-inflammatory, anti-tumour and anti-germ tube formation protection and other cytotoxic activities. This compound has pharmacological properties such as HIV inhibition, in the food sector, mono-ammonium salt (glycyrram) is a form of sweetener with nutrient enhancement properties. The present study experiments on methods for Development of somatic embryos from root culture and to regenerate plant using root cultures. The observations revealed that, shoot initiation and rate of root growth ranged from 65.4% to 89.3% while plant regeneration from somatic embryos was 82.6% when cultures in 1/4th strength Murashige and Skoog (MS) medium by sucrose 2% used as a supplement. The study illustrates that proliferative germination of seeds in respective media and also suggested that T. cuneifolia could be used as substitute of G. Glabra used as an systematic methodology and plants are mass-produced from a single mother plant.