Glycyrrhizic acid attenuated lipid peroxidation induced by titanium dioxide nanoparticles in rat liver.

Abstract

to investigate the hepatoprotective effect of glycyrrhizic acid (GA) against hepatic injury induced by titanium dioxide nanoparticles (NTiO2) in rats. Many recent studies demonstrate that most nanoparticles (NPs) have an adverse or toxic action on liver. NTiO2- intoxicated rats received 300 mg/kg of NTiO2 for 14 days by gavage method. Protection group was pretreated with 10 mg/kg of GA for 7 days before NTiO2 administration. Alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (ALP) were detected as biomarkers in the blood to indicate hepatic injury. Product of lipid peroxidation (MDA), superoxide dismutase (SOD) and glutathione peroxidase (GPx) were evaluated for oxidative stress in hepatic injury. Light microscopy for histopathological studies was also done. Administration of NTiO2 induced a significant elevation in plasma AST, ALT and ALP. In the liver, NTiO2 increased the oxidative stress through the increase in lipid peroxidation and decrease in SOD and GPx enzymes. Pretreatment of GA significantly decreased ALT, AST and ALP, attenuated the histopathology of hepatic injury, ameliorated oxidative stress in hepatic tissue, and increased the activities of SOD and GPx. These findings indicate that GA effectively protects against NTiO2-induced hepatotoxicity in rats and might be clinically useful (Fig. 4, Ref. 47). Text in PDF www.elis.sk.