Glycosylation of Polyphosphazene Nanofibrous Membrane by Click Chemistry for Protein Recognition

Abstract

AbstractA novel core/sheath glycosylated polyphosphazene nanofibrous membrane for use in protein recognition is fabricated via coaxial electrospinning process with alkyl polyphosphazene as the sheath and polyacrylonitrile (PAN) as the core, followed by introduction of saccharide residues to the surface of the polyphosphazene nanofibrous membrane using alkyne–azide “click” chemistry. A glucose/protein binding assay effectively demonstrates that this nanofibrous membrane can selectively recognize Con A while showing almost no binding with bovine serum albumin (BSA). Furthermore, the affinity capability of proteins (BSA and Con A) for the glycosylated polyphosphazene surface is investigated quantitatively using a surface plasmon resonance (SPR) technique.magnified image