Abstract
BackgroundIron in the overloaded condition in liver promotes the overproduction of free radicals that lead to oxidative stress and ultimately hepatic damage. The present study was designed to evaluate the ameliorating potential from iron overloaded hepatotoxicity by the glycosidic fraction from Spondious pinnata bark (SPW1) along with its antioxidant property.MethodsThe fraction was tested for its in vitro antioxidant, free radical scavenging property and iron chelation potential via standard biochemical assays. Iron overload condition was generated by the intraperitoneal administration of iron dextran in mice. The levels of serum enzymes, antioxidant enzymes in liver, markers of hepatic damage, liver iron, and ferritin content were measured in response to the oral treatment of SPW1. Histopathology of the liver sections was performed for visual confirmation of the amelioration potential of SPW1.ResultsThe fraction exhibited excellent in vitro antioxidant as well as free radical scavenging potential against both reactive oxygen species and reactive nitrogen species. Administration of SPW1 significantly normalized the disturbed levels of antioxidant enzymes, liver iron, lipid peroxidation, liver fibrosis, serum enzyme and ferritin better than standard desirox which were also supported by the morphological study of the liver sections. Phytochemical analysis as well as HPLC study, confirmed that the fraction mainly consisted of glycosidic phenolics and flavonoids that attributed to its biological activities.ConclusionsThe above results suggested that beneficial effects of SPW1 on iron overload induced hepatotoxicity that can be considered as a possible candidate against iron overload diseases.
Highlights
Iron in the overloaded condition in liver promotes the overproduction of free radicals that lead to oxidative stress and hepatic damage
In vitro study Antioxidant potentials The overall picture of the antioxidative ability of SPW1 was evaluated by ABTS+ radical cation scavenging, DPPH radical scavenging and reducing power capacity assays
It was observed that SPW1 possessed excellent total antioxidant capacity that is almost similar to the standard Trolox (Fig. 1a)
Summary
Iron in the overloaded condition in liver promotes the overproduction of free radicals that lead to oxidative stress and hepatic damage. Different phytochemicals mainly natural water soluble phenolics and flavonoids are used to treat iron-induced liver toxicity as they can efficiently scavenge most of the free radicals through their relevant iron chelating capabilities. F.) Kurz (Fam. Anacardiaceae) possessed both in vitro & in vivo antioxidant and iron-chelating potential, which was supported by the presence of significant amounts of phenolics and flavonoids [5, 6]. Anacardiaceae) possessed both in vitro & in vivo antioxidant and iron-chelating potential, which was supported by the presence of significant amounts of phenolics and flavonoids [5, 6] These previous studies prompted us to separate the water soluble glycosidic compounds from S. pinnata bark and evaluate their ameliorating effect on iron overload-induced hepatotoxicity and hepatic fibrosis in mice
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
