Abstract
Phytochemicals of various origins are of great interest for their antidiabetic potential. In the present study, the inhibitory effects against carbohydrate digestive enzymes and non-enzymatic glycation, antioxidant capacity, and phenolic compounds composition of Viburnum opulus L. fruits have been studied. Crude extract (CE), purified extract (PE), and ethyl acetate (PEAF) and water (PEWF) fractions of PE were used in enzymatic assays to evaluate their inhibitory potential against α-amylase with potato and rice starch as substrate, α-glucosidase using maltose and sucrose as substrate, the antioxidant capacity (ABTS, ORAC and FRAP assays), antiglycation (BSA-fructose and BSA-glucose model) properties. Among four tested samples, PEAF not only had the highest content of total phenolics, but also possessed the strongest α-glucosidase inhibition, antiglycation and antioxidant activities. UPLC analysis revealed that this fraction contained mainly chlorogenic acid, proanthocyanidin oligomers and flavalignans. Contrary, the anti-amylase activity of V. opulus fruits probably occurs due to the presence of proanthocyanidin polymers and chlorogenic acids, especially dicaffeoylquinic acids present in PEWF. All V. opulus samples have an uncompetitive and mixed type inhibition against α-amylase and α-glucosidase, respectively. Considering strong anti-glucosidase, antioxidant and antiglycation activities, V. opulus fruits may find promising applications in nutraceuticals and functional foods with antidiabetic activity.
Highlights
Type 2 diabetes mellitus (T2DM) is a global problem because it concerns hundreds millions of people and has a significant impact on the health, quality of life and the health care system [1]
The inhibitory effects of different samples separated from V. opulus dried fruits such as crude extract (CE), purified extract (PE), and two fractions separated from PE, called water fraction (PEWF) and ethyl acetate fraction (PEAF), were analyzed by the dose-effect plots (Supplementary Figure S1)
The potential ability of V. opulus dried fruit extracts, especially enriched with phenolic compounds to inhibit α-amylase and α-glucosidase, and advanced glycation-end products (AGE) formation, and exhibition of antioxidative activities could be some of antidiabetic properties
Summary
Type 2 diabetes mellitus (T2DM) is a global problem because it concerns hundreds millions of people and has a significant impact on the health, quality of life and the health care system [1]. The antidiabetic activity of plant-derived foods and extracts has been attributed, inter alia, to the naturally available phenolic compounds [6,12,13,14,15,16,17,18,19]. These secondary plant metabolites comprise one (phenolic acids such as hydroxybenzoic and hydroxycinnamic acids) or more (polyphenols containing flavonoids, tannins, lignans) aromatic rings with attached hydroxyl group in their structures [20]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
