Glycoproteins released by Leishmania donovani: immunologic relationships with host and bacterial antigens and preliminary biochemical analysis.

Abstract

The antigenically active glycoproteins (AAGP) released by Leishmania donovani strain 3S promatigotes into growth media and by amastigotes of this strain into the tissue, e.g. blood, of infected hamsters was found to consist of 6 to 7 antigenically distinct components. The antigenic activity of these glycoproteins was resistant to freeze-thawing, protease treatment, and purification by column chromatography using Sephadex G-100. This activity, however, was destroyed by Na periodate and altered by boiling; AAGP adhered firmly to Amicon filter (UM2). The antigenically active substances absorbed UV at 230, 260, 280 nm and gave positive Folin phenol, phenol sulfuric acid, and orcinol reactions. By gel diffusion, the component glycoproteins were found to form lines with concanavalin A and to give reactions to identity and partial identity with human red cells and Mycobacterium butyricum. The possible involvement of the antigenically active glycoproteins in pathogenesis of kala azar is discussed.