Glycomyces salinus sp. nov., an actinomycete isolated from a hypersaline habitat.

Rui Li,Shu-Kun Tang,Gang-Qiang Jiang,Yun Wang,En-Min Zhou,Yi-Guang Chen

doi:10.1007/s00203-021-02510-4

Rui Li, Shu-Kun Tang + Show 4 more

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https://doi.org/10.1007/s00203-021-02510-4

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Abstract

A Gram-stain-positive, aerobic, nonmotile actinobacterium, designated strain YIM 93776T, was isolated from a saline sediment sample collected from Aiding Lake in Xinjiang Uygur Autonomous Region, Northwest China. Phylogenetic analysis based on the 16S rRNA gene sequences showed that strain YIM 93776T was affiliated to the genus Glycomyces and was closely related to Glycomyces albus TRM 49136T (97.6% sequence similarity), Glycomyces lacisalsi XHU 5089T (97.0%) and Glycomyces anabasis EGI 6500139T (96.2%). The cell wall contained meso-diaminopimelic acid and the whole-cell hydrolysates sugars were galactose, mannose, arabinase, glucose and ribose. The predominant menaquinones were MK-9 (H4) and MK-10 (H4). Diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, two phosphatidylglyceride, two unidentified phospholipids and two unidentified polar lipids were detected in the polar lipid extracts. Major fatty acids were anteiso-C17:0, iso-C15:0, iso-C16:0, anteiso-C15:0 and anteiso C17:1 A. The draft genome sequence of strain YIM 93776T was 5.37 Mbp in size with 69.5% DNA G + C content. The dDDH and ANI values between strain YIM 93776T and related neighbours were 25.0-34.3% and 77.3-79.8%, respectively. On the basis of morphological, chemotaxonomic and phylogenetic evidence, strain YIM 93776T; therefore, represents a novel species, for which the name Glycomyces salinus sp. nov. is proposed. The type strain is YIM 93776T (= KCTC 49430T = CGMCC 4.7685T).

Highlights

The genus Glycomyces was initially proposed by Labeda et al (1985) with Glycomyces harbinensis as the type species, and the description was later emended by Labeda and Kroppenstedt (2004)
At the time of writing, there are 26 validly published names in this genus with the latest described Glycomyces terrestris by Li et al (2021), Glycomyces albidus by Qian et al (2020), Glycomyces buryatensis by Nikitina et al (2020), Glycomyces xiaoerkulensis by Wang et al (2018), Glycomyces sediminimaris by Mohammadipanah et al (2018), Glycomyces paridis by Fang et al (2018), Glycomyces dulcitolivorans by Mu et al (2018), and Glycomyces anabasis by Zhang et al (2018)
We isolated a halophilic actinobacteria designated as strain YIM 93776T that was associated with genus Glycomyces

Summary

Introduction

The genus Glycomyces was initially proposed by Labeda et al (1985) with Glycomyces harbinensis as the type species, and the description was later emended by Labeda and Kroppenstedt (2004). Phylogenetic analysis based on the 16S rRNA gene sequences showed that strain YIM 93776T was affiliated to the genus Glycomyces, and was closely related to Glycomyces albus TRM 49136T (97.6 % sequence similarity), Glycomyces lacisalsi XHU 5089T (97.0 %) and Glycomyces anabasis EGI 6500139T (96.2 %). On the basis of morphological, chemotaxonomic and phylogenetic evidence, strain YIM 93776T represents a novel species, for which the name Glycomyces salinus sp.

Results

Conclusion