Glycolytic enzyme levels of intraocular fluids and lens as compared to the respective sera of various animal species.

Abstract

The levels of glycolytic enzymes, aldolase, phosphohexose isomerase and lactic dehydrogenase, the latter as total and isozymes, were determined in the vitreous and aqueous humors and sera as well as in lens-saline extracts of the cat, Rhesus monkey, guinea pig, rabbit, rat and cattle. Although wide species differences were noted, the levels were generally lower in the aqueous as compared to the vitreous and serum. Aldolase and phosphohexose isomerase were invariably elevated in the lens of most species. The LDH isozyme patterns were quite unique in regard to the fluids and the species. The ratios of enzyme contents of the intraocular fluids to the respective sera were calculated and the findings compared. As tested in a few species, no remarkable enzyme differences could be discerned as a result of prior dark or light adaptation of the eyes.