Abstract
The effects of transformation by murine sarcoma virus and of increasing cell density on the activities of several key glycolytic enzymes in Balb 3T3 cells were tested. Hexokinase levels increased with culture density in the uninfected and in the two virus-transformed (HB2 and KA31) cells. Phosphofructokinase did not increase with culture density in the uninfected cells but rose dramatically in dense cultures of virus-transformed cells. 6-Phosphogluconate dehydrogenase and glucose-6-phosphate dehydrogenase levels were high in sparse cultures of uninfected cells and decreased steadily with increased culture density. Pyruvate kinase levels increased with density only in KA31 cultures. A density-dependent decrease in the level of hexokinase type II with a concomitant increase in type I isozyme was seen in uninfected 3T3 cultures. This change was negligible in HB2 cells.
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