Abstract
Bis-quaternary ammonium salts (H25C12-CH3-N-CH3-CmH2m-CH3-N-CH3-C12H25 m : 220) were found to form vesicles as molecular aggregates. The vesicles were used as reaction media to evaluate the activity of a hydrolase model ; the hydrolysis of amino acid esters was carried out using a glycolipid as a model of α-chymotrypsin. Catalytic activity was affected by vesicle membrane fluidity and the addition of cholesterol caused the membrane to become somewhat rigid. Essentially the same is noted for reactions in a living system.
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