Glycogen from Klebsiella pneumoniae M 5 al and Escherichia coli K 12

Abstract

1. A continuous two stage cultivation method for two strains of Klebsiella pneumoniae and Escherichia coli yielding high cell mass and relatively high glycogen contents is described. The stage 1 cells (carbon-limited) were fed with the nitrogen source ammonia (which also neutralized simultaneously) soly via the pH-stat. In stage 2, the cells grew nitrogen-limited, a small excess of the carbon source was maintained by continuous addition of a glucose solution.