Glycodelin Effect on the Spleen State in Case of Allogenous Bone Marrow Cell Transplantation in the in Vivo Experiment

Abstract

Glycodelin, a protein associated with pregnancy, has an immunosuppressive effect and is promising for use in biomedicine. The aim of this work was to investigate the effect of recombinant glycodelin (MyBioSource, Germany) on the morphofunctional state of the spleen in case of allogeneic transplantation of red bone marrow cells in Wistar rats in a dynamic experiment in vivo. In the intact group, animals received a suspension of bone marrow cells treated once with camptothecin; in the experimental group, in addition to the introduction of the same bone marrow cells, the animals received 4 injections of glycodelin. Glycodelin was shown to promote the activation of immune system cells in the spleen against the background of allogeneic bone marrow transplantation and to stimulate the proliferation (Ki-67) and differentiation of immune cells. However, the number of macrophages (CD68) is reduced under the influence of glycodelin. As a result, glycodelin may affect the development of adaptive responses in bone marrow transplantation. Apparently, glycodelin is able to participate in the development of an adaptive mechanism in the spleen.