Glycine betaine capped ZnO NPs eliminate oxidative stress to coriander plants grown under NaCl presence

Abstract

Salinity is one of the major abiotic stresses for sustainable agriculture. The use of mineral nutrients in form of nanoparticles can be a novel strategy to fight against abiotic stresses. An in vitro study has been conducted to investigate the effect of zinc oxide nanoparticles (ZnO NPs) capped with glycine betaine (ZnOBt) on coriander plants exposed to saline (NaCl) stress. SEM and XRD analysis revealed 14.73 nm and 17.34 nm size of ZnO and ZnOBt NPs, respectively with spherical to hexagonal structures. Coriander plant length and biomass increased by the application of ZnO and ZnOBt NPs. ZnOBt NPs depicted promising results at 100 mg/L where, shoot and root length increased up to 14 cm and 13 cm, respectively as compared to plants grown under saline stress. ZnOBt NPs also increased fresh and dry weight of shoots and roots as compared to other treatments. The results depict that ZnOBt NPs mitigated stress condition. This is evident from concentration of phenolic and flavonoid contents that decreased in both roots and shoots. Free radical scavenging activity, total antioxidant capacity and total reducing power also decreased in plants by ZnOBt NPs when applied with stress. The concentration of superoxide and peroxide dismutase also decreased by application of ZnOBt NPs to salt stress plants. Glycine betaine with ZnO NPs, in conclusion, can be an effective remedy for salinity-exposed plants. These nanoparticles can be encouraged as a viable technique to overcome the detrimental effects of saline stress on plants.