Abstract
1. Aminoacyl tRNA synthetase was extracted from the silkgland of silkworm (Bombyxmori Linne) and fractionated on a DEAE-cellulose column. Activities were estimated by ATP-PPi exchange reaction as well as glycyl tRNA formation.2. Two peaks, A and B, having ATP-PPi exchange activity were found in the separated fractions, respectively. There was also observed a marked difference between the both peaks with respect to the pH optimum and activity dependence on MgCl2 concentration.3. Peak A showed no activity of glycyl tRNA formation. Only a part of peak B coincided with the activity of glycyl tRNA formation. The activities of both the ATP-PPi exchange reaction and glycyl tRNA formation were found to be dependent on MgCl2 concentration, and the optimum concentration was different between two peaks.4. It also seemed to exist two peaks of activities, a and b, in glycyl tRNA formation which could be separated with a DEAE-cellulose column.
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