Abstract
Human Acellular Dermal Matrices (HADM) are employed in various reconstructive surgery procedures as scaffolds for autologous tissue regeneration. The aim of this project was to develop a new type of HADM for clinical use, composed of glycerolized reticular dermis decellularized through incubation and tilting in Dulbecco’s Modified Eagle’s Medium (DMEM). This manufacturing method was compared with a decellularization procedure already described in the literature, based on the use of sodium hydroxide (NaOH), on samples from 28 donors. Cell viability was assessed using an MTT assay and microbiological monitoring was performed on all samples processed after each step. Two surgeons evaluated the biomechanical characteristics of grafts of increasing thickness. The effects of the different decellularization protocols were assessed by means of histological examination and immunohistochemistry, and residual DNA after decellularization was quantified using a real-time TaqMan MGB probe. Finally, we compared the results of DMEM based decellularization protocol on reticular dermis derived samples with the results of the same protocol applied on papillary dermis derived grafts. Our experimental results indicated that the use of glycerolized reticular dermis after 5 weeks of treatment with DMEM results in an HADM with good handling and biocompatibility properties.
Highlights
Human Acellular Dermal Matrices (HADM) provide a clean scaffold for host cellular and vascular in-growth
We describe a new process for HADM production, based on the use of pure reticular dermis (RD) preserved by glycerol, in order to obtain dermal grafts suitable for clinical use
Several methods of producing HADM are described in the literature, distinguished in terms of preservation, tissue origin and characteristics and decellularization procedures [17,20,22,24,25]
Summary
Human Acellular Dermal Matrices (HADM) provide a clean scaffold for host cellular and vascular in-growth. They are used in various reconstructive surgery procedures, for tissue regeneration in full thickness burns and for suspension, reinforcement and ligamentous restoration in breast reconstruction, abdominal wall repair and capsular repair in orthopedic surgery, PLOS ONE | DOI:10.1371/journal.pone.0149124. Production of a New Human Acellular Dermal Matrix study design, data collection and analyses, decision to publish, or preparation of the manuscript They are used in various reconstructive surgery procedures, for tissue regeneration in full thickness burns and for suspension, reinforcement and ligamentous restoration in breast reconstruction, abdominal wall repair and capsular repair in orthopedic surgery, PLOS ONE | DOI:10.1371/journal.pone.0149124 February 26, 2016
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