Abstract
Objectives To assess glycerol as reference material for low-resolution time-domain 1H NMR analysis of fecal fat. Design and methods NMR analysis of fecal fat in stool samples with added glycerol was used to assess linearity, recovery, and relationship with NMR lipid signal. Results The study revealed for added glycerol excellent linearity (r = 0.9998), recovery (101–104%), and linear relationship with simulated fecal fat content. Conclusions Glycerol is an effective reference material for NMR fecal fat analysis.
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