Glycerol as a new dissolution medium for α-, β- and γ-cyclodextrins for preparing stereoselective stationary phases for gas-liquid chromatography

Abstract

Glycerol was applied as the dissolution medium for α, β- and γ-cyclodextrins and its gas-liquid chromatographic performance was compared with that of formamide. Various stationary glycerol-based chiral phases for classical gas-liquid chromatography were examined. The columns containing glycerol appeared relatively stable in comparison with those prepared with formamide. Glycerol-based phases functioned over the temperature ranged 80–100°C for about 400 h without any changes indicating losses of glycerol or activity. Owing to the lower stability of cyclodextrin complexes in glycerol medium relative to those in formamide, and the fact that glycerol-based columns allow operation ca. 40°C higher, many separations which were previously unreported at higher temperatures were achieved. The model compounds tested were (+/−)-α-pinene, cis/trans-decalin, cis/trans-anethole, cis/trans-isosafrol, cis/trans-isoeugenol, (+/−)-camphor, (+/−)-fenchone, (+/−)-isomenthone, (+/−)-isoponocampheol, (+/−)-borneol and (+/−)-isoborneol.