Glycerol and Antimicrobial Peptide-Modified Natural Latex for Bacteriostasis of Skin Wounds.

Abstract

This work aimed to develop a glycerol antimicrobial peptide natural latex film (NRL-GI-AMP film) for the treatment of skin wound infections. The contents of this work mainly include investigating the effect of adding glycerol (GI) and an antimicrobial peptide (AMP) on the physical and chemical properties of natural latex (NRL) and analyzing the cytocompatibility, bacteriostatic activity, and infected wound healing promotion of the NRL-GI-AMP film. The results showed that the addition of GI resulted in more pores in the internal structure of the NRL film, while the addition of G(LLKK)3L AMP did not change the structure and properties of the NRL film. Compared with that of the NRL film, the infrared spectrum of the NRL-GI-AMP film did not produce new characteristic peaks, indicating that GI and AMP were non-covalently cross-linked with NRL. Addition of 10% GI reduces the toughness of the NRL-GI-AMP film by 62.0%, increases the water vapor transmission rate by 8.95 mg/(cm2·h), and reduces the water absorption and water retention distributions by 33.0 and 24.7%, respectively. AMP in the NRL-GI-AMP film could be released continuously for 40 h, and the release rate was about 45%. The NRL-GI-AMP film showed good biocompatibility and antibacterial activity and promoted the healing of infected wounds. Therefore, the NRL-GI-AP film has potential application in the development of dressings to inhibit skin wound infection and promote wound healing.