Glycation of benign meningioma cells leads to increased invasion.

Philipp Selke,Maximilian Scheer,Christian Strauss,Philip Rosenstock,Rüdiger Horstkorte,Kaya Bork

doi:10.1515/hsz-2020-0376

Philipp Selke, Maximilian Scheer + Show 4 more

Open Access

https://doi.org/10.1515/hsz-2020-0376

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Abstract

Meningiomas are the most common non-malignant intracranial tumors. Like most tumors, meningiomas prefer anaerobic glycolysis for energy production (Warburg effect). This leads to an increased synthesis of the metabolite methylglyoxal (MGO). This metabolite is known to react with amino groups of proteins. This reaction is called glycation, thereby building advanced glycation endproducts (AGEs). In this study, we investigated the influence of glycation on two meningioma cell lines, representing the WHO grade I (BEN-MEN-1) and the WHO grade III (IOMM-Lee). Increasing MGO concentrations led to the formation of AGEs and decreased growth in both cell lines. When analyzing the influence of glycation on adhesion, chemotaxis and invasion, we could show that the glycation of meningioma cells resulted in increased invasive potential of the benign meningioma cell line, whereas the invasive potential of the malignant cell line was reduced. In addition, glycation increased the E-cadherin- and decreased the N-cadherin-expression in BEN-MEN-1cells, but did not affect the cadherin-expression in IOMM-Lee cells.

Highlights

Meningioma represents the most common non-malignant intracranial tumor (Goldbrunner et al 2016; Holleczek et al 2019; Ostrom et al 2018, 2019).Like many tumors, meningiomas need large amounts of glucose as primary energy source, because they mainly metabolize glucose to lactate during anaerobic glycolysis (Warburg effect) (Bharadwaj et al 2015), which generates only low amounts of adenosine triphosphate (ATP)
We investigated the influence of glycation on two meningioma cell lines, representing the WHO grade I (BEN-MEN-1) and the WHO grade III (IOMM-Lee)
Little is known about the role of MGO and glycation on meningioma cells

Summary

Introduction

Meningiomas need large amounts of glucose as primary energy source, because they mainly metabolize glucose to lactate during anaerobic glycolysis (Warburg effect) (Bharadwaj et al 2015), which generates only low amounts of adenosine triphosphate (ATP). This changed (anaerobic) energy metabolism is one of the “hallmarks of cancer” (Gill et al 2016). There are many inconsistent data suggesting a positive (Schneider et al 2005; Schwartzbaum et al 2005) or inverse (Bernardo et al 2016) relationship between diabetes and serum glucose levels and the risk of meningioma. Patients with type 2 diabetes have a decreased survival after surgical resection of a WHO grade I meningioma (Nayeri et al 2016)

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