Abstract
ABSTRACT The objective of this study was to evaluate the rate of conception, metabolic, and structural conditions of cryopreserved bovine sperm cells, plus extender with IGF-1 and glutathione (GSH). 12 ejaculations of Nelore bulls were used, submitted to treatments: control, gSH (2mM/mL), IGF-1 (100ng/mL) and gSH (1mM/mL) + IGF-1 (50ng/mL). After cryopreservation and thawing the semen passed the fast thermo resistance test (TTR), plasma membrane and acrosomal integrity (PIAI), mitochondrial membrane potential (AP), oxidative stress, and conception rate. Tukey test was used for the statistical analysis of the parametric variables and the Friedman test for nonparametric. The gestation percentage was compared by the Chi-square test. There was no statistical difference (P<0.05) between treatments for the TTRr variable. Otherwise in the oxidative stress evaluated with the CellROX probe was noted that the IGF-1 showed the highest number of reactive cells (P<0.05). The PIAI, AP and gestation rate showed no difference among treatments (P>0.05), with an average of conceptions of 36.58%. It is concluded that IGF-1, gSH and their association did not cause changes in sperm motility, mitochondrial potential, plasma and acrosomal membrane integrity. IGF-1 increased oxidative stress, however, there was no difference in the gestation rate among the treatments.
Highlights
Seminal plasma is a complex mixture of secretions originated in the testicles, epididymides, and accessory sexual glands (Manjunath et al, 1993), serving as a vehicle for ejaculated sperm, in addition to containing factors that influence their fertilization capacity (Calvete et al, 1996)
According to the review by Kumar et al (2019), IGF-I supplementation improves semen quality in several species, such as dogs, stallions, and bulls, and their protective function may be due to antioxidant activities (Selvaraju et al, 2016), which is diminished or lost in the process of handling seminal plasma that routinely require dilution, reducing the concentration of these, which results in cell stress (Bilodeau et al, 2000)
Another antioxidant that is lost in this seminal dilution is glutathione (GSH) which is responsible for the reconstruction of thiol groups (-SH) in proteins, which can be eliminated during oxidative stress, as well as protecting cell membranes from lipid oxidation preventing the formation of free oxygen (Lenzi et al, 1994)
Summary
Seminal plasma is a complex mixture of secretions originated in the testicles, epididymides, and accessory sexual glands (Manjunath et al, 1993), serving as a vehicle for ejaculated sperm, in addition to containing factors that influence their fertilization capacity (Calvete et al, 1996). According to the review by Kumar et al (2019), IGF-I supplementation improves semen quality in several species, such as dogs, stallions, and bulls, and their protective function may be due to antioxidant activities (Selvaraju et al, 2016), which is diminished or lost in the process of handling seminal plasma that routinely require dilution, reducing the concentration of these, which results in cell stress (Bilodeau et al, 2000) Another antioxidant that is lost in this seminal dilution is glutathione (GSH) which is responsible for the reconstruction of thiol groups (-SH) in proteins, which can be eliminated during oxidative stress, as well as protecting cell membranes from lipid oxidation preventing the formation of free oxygen (Lenzi et al, 1994). A great number of studies propose that the production of reactive oxygen species (ROS) and the semen's oxidative capacity enhance the harmful effects of seminal manipulation (Bustamante Filho et al, 2006)
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